Journal: Communications Biology
Article Title: Synthetic indole derivatives as an antibacterial agent inhibiting respiratory metabolism of multidrug-resistant gram-positive bacteria
doi: 10.1038/s42003-024-06996-8
Figure Lengend Snippet: A Evolution of resistant mutants of SMJ-2 (blue) and ciprofloxacin (brown) in cation-adjusted broth on S. aureus ATCC 29213, B S. aureus ATCC 43300, and ( C ) B. subtilis MTCC 121. Cells were passaged every 24 h. D , E Time-kill kinetic assay of SMJ-2 and SMJ-4 on S. aureus ATCC-MRSA-43300 in logarithmic and stationary phase respectively, the concentration of SMJ-2, SMJ-4, vancomycin, linezolid, and oxacillin at 10 × MIC. MIC of SMJ-2, SMJ-4, vancomycin, linezolid, and oxacillin were 2, 4, 1, 2, and 64 µg mL −1 respectively; (L.O.D. = Limit of Detection), F Photographic representation of SMJ-2, SMJ-4, vancomycin and linezolid treatment on S. aureus ATCC-MRSA-43300 in logarithmic phase, G Oxacillin treatment to S. aureus ATCC-29213 form persister cells, H Persister killing assay in the presence of SMJ-2 at 5 × MIC, 10 × MIC, and 20 × MIC and linezolid was used as positive control at 10 × MIC, and 20 × MIC. Experiments ( A – C ) represent two individual repeats ( n = 2 biologically independent experiments), D , E , G , and H represent three individual repeats ( n = 3 biologically independent samples), and the results correspond to average ± standard deviation.
Article Snippet: A Evolution of resistant mutants of SMJ-2 (blue) and ciprofloxacin (brown) in cation-adjusted broth on S. aureus ATCC 29213, B S. aureus ATCC 43300, and ( C ) B. subtilis MTCC 121.
Techniques: Kinetic Assay, Concentration Assay, Positive Control, Standard Deviation